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. 2014 May 21;9(5):e97624. doi: 10.1371/journal.pone.0097624

Figure 3. microRNAs targeting THRB transcript.

Figure 3

A. The effect of microRNA mimics on the activity of luciferase expressed from a reporter plasmid pGL3-luc-3′UTR-THRB. HeLa cells were transfected using the reporter plasmid and respective microRNA mimics: pre-miR-155, pre-miR-425, pre-miR-599, or pre-miR-221. The results are shown as percentage of control (Ctrl, cells transfected with non-targeting pre-miR). The plot shows results of three independent biological experiments, measured in triplicates. The relative activity of Firefly luciferase was normalized to Renilla luciferase activity. Statistical analysis was performed using ANOVA followed by Dunnett’s multiple comparison test. *** p<0.001. B. The effect of miR-155 and miR-425 on endogenous THRB expression in renal cancer cells. UOK171 cells were transfected using respective microRNA mimics (pre-miRs) or inhibitors (anti-miRs), and THRB mRNA expression was analyzed using real-time PCR. The results are shown as percent of control (the cells transfected with non-targeting pre-miR). The plots shows results of three independent biological experiments measured in triplicates. Statistical analysis was performed using ANOVA followed by Dunnett’s multiple comparison test. *p<0.05, *** p<0.001. C. The expression of miR-155 and miR-425 in nontumorous controls (C, n = 35) and paired ccRCC tissue samples (T, n = 35). The results are shown as percent of control. Real-time PCR for each sample was performed in triplicates. Statistical analysis was performed using Wilcoxon matched pair test. *p<0.05, **p<0.01.