Table 1. Nucleotide sequence of the primers and probes used in conventional multiplex PCR, multiplex real-time PCR.

Primer or probe name	Sequence (5′→3′)	5′ Reporter dye 3′ Quencher
16S rRNA*
Staph-756F	AAC TCT GTT ATT AGG GAA GAA CA
Staph-750R	CCA CCT TCC TCC GGT TTG TCA CC
nuc †
nuc For	CAA AGC ATC AAA AAG GTG TAG AGA
nuc Rev	TTC AAT TTT CTT TGC ATT TTC TAC CA	Texas Red
nuc Probe	TTT TCG TAA ATG CAC TTG CTT CAG GAC CA	Iowa Black
mecA
mecA-1F*	GTA GAA ATG ACT GAA CGT CCG ATA A
mecA-2F*	CCA ATT CCA CAT TGT TTC GGT CTA A
mecA For†	GGC AAT ATT ACC GCA CCT CA
mecA Rev†	GTC TGC CAC TTT CTC CTT GT	FAM†
mecA Probe†	AGA TCT TAT GCA AAC TTA ATT GGC AAA TCC	TAMRA†
PVL
luk-PV-1F*	ATC ATT AGG TAA AAT GTC TGG ACA TGA TCC A
luk-PV-2R*	GCA TCA AGT GTA TTG GAT AGC AAA AGC
PVL For†	ACA CAC TAT GGC AAT AGT TAT TT
PVL Rev†	AAA GCA ATG CAA TTG ATG TA	Cy5†
PVL Probe†	ATT TGT AAA CAG AAA TTA CAC AGT TAA ATA TGA	Iowa Black

*Conventional multiplex PCR, according to McClure et al. (2006) [43].

^†Multiplex real-time PCR, according to McDonald et al. (2005) [35].