Figure 2. K16ApoE-mediated brain delivery of blue (EB), red (Crocein Scarlet) and green (Light Green SF) dyes to the brain.
Three different approaches were assessed for dye delivery: 1. K16ApoE was injected first then a given dye was injected 10 min after (second columns of brain specimens); 2. K16ApoE was mixed with 300 ug of cetuximab and injected followed by injection of a given dye 10 min after 3rd column of brain specimens), and 3. K16ApoE and the dyes were mixed and injected (fourth column of brain specimens). The first column of brain specimens represents animals receiving injection of a given dye alone. Mice were perfused with saline 2 h after injection and then brains were collected for visualization. 67.5 picomole of K16ApoE was used in each experiment. 40 ul of a 2% solution of each of the dyes were used for injection into a 20 g mouse (amount of dye injected varied accordingly with weight of mice).