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. 2014 May 21;9(5):e97781. doi: 10.1371/journal.pone.0097781

Figure 1. Metformin did not inhibit colorectal cancer cell growth and induce apoptosis or autophagy.

Figure 1

(A) HCT116, RKO and HT29 were seeded in 96-well plates. After 24 h, metformin (1 and 5 mM) and AICAR (5 mM) were added to the culture media. 24 h after the addition of the agents, the effect of metformin on colorectal cancer cell survival was performed a cell viability assay (MTT). (B) Photographs of soft agar colonies of HCT116, RKO and HT29 cells 2 weeks after the treatment with 5 mM metformin and 5 mM AICAR. (3) Cancer cell lines were treated with different concentrations of metformin (1, 5 and 10 mM) for various time, then, the expression of active caspase-3 and LC3-II/I ratio was assessed by western blotting.