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. 2014 May 21;9(5):e97781. doi: 10.1371/journal.pone.0097781

Figure 2. Metformin did not induce apoptosis and cell cycle arrest.

Figure 2

(A) 24 h after the addition of 5 mM metformin and 5 mM AICAR, the effect of metformin on colorectal cancer cell survival was observed. Representative cell morphological changes detected by light microscopy; characteristic morphological features of apoptosis were observed, including detachment and cell shrinkage. (B) Fractions of cells in the sub-G1, G0/G1, S or G2/M phases of the cell cycle are investigated after the treatment with metformin and AICAR.