Table 1. Grouping of animals and number of animals per group and testing parameter.
Groups | MRI# | SSEP# | Sensorimotor function# | IHC/ LFB | Protein Expression (WB) | |||
Time | day | day | day | day | day | |||
continuous | continuous | continuous | 22 | 4 | 10 | 16 | 22 | |
TBI | 6* | 12 | mTBI, 9* sTBI, 12 | 6 | 5 | 5 | 5 | 5 |
TBI+ HBOT | 10* | 12 | mTBI, 9* sTBI, 12 | 6 | 5 | 5 | 5 | 5 |
Sham | - | - | 7 | 6 | 5 | 5 | 5 | 5 |
*Uneven number of animals is due to trauma- or anaesthesia-related loss of rats. #Different groups were implemented since this allowed for simultaneous analysis of these parameters. Biases in the results of the distinct testing parameters due to previous handling of the animals were thereby avoided, i.e. anaesthetics and interventions during MRI or SSEP did not affect behavioural potentials of the rats and vice versa. mTBI: moderate traumatic brain injury; sTBI: severe traumatic brain injury.