Table 2.

Immunoreactivity of rV- neu T vaccinated BALB- neu T mouse sera with Neu

Dose of rV- neu T	Number of mice with immune response/total a	Number of pooled sera	Serum titer (SD)b
108 pfu	8/8	5	11875c (1250) p = 0.000288d p = 0.0045e
107 pfu	4/4	4	1950 (1706)
106 pfu	6/6	5	3380 (3750)

^aImmune response was determined by ELISA against LTR-Neu and NIH3T3. Optical density (O.D) of V-wt sera at 1:250 to LTR-Neu was ≤0.3. These values were considered negative. Values for rV-neuT serum was considered positive when specific O.D value was ≥0.5 at the 1:1000 dilution.

^bImmune sera titers of BALB-neuT vaccinated mice were determined by ELISA against LTR-Neu and NIH3T3 using individual serum at different dilutions (1:1000, 1:2500, 1:12500). Sera titer represents the mean value of individual serum titer ± standard deviation (SD).

^cTiter was estimated as the highest immune serum dilution generating a specific absorbance of 0.5 at 492 nm.

^d10⁸ pfu vs 10⁷ pfu.

^e10⁸ pfu vs 10⁶ pfu.