Table 2.
Dose of rV- neu T | Number of mice with immune response/total a | Number of pooled sera | Serum titer (SD)b |
---|---|---|---|
108 pfu |
8/8 |
5 |
11875c (1250) p = 0.000288d p = 0.0045e |
107 pfu |
4/4 |
4 |
1950 (1706) |
106 pfu | 6/6 | 5 | 3380 (3750) |
aImmune response was determined by ELISA against LTR-Neu and NIH3T3. Optical density (O.D) of V-wt sera at 1:250 to LTR-Neu was ≤0.3. These values were considered negative. Values for rV-neuT serum was considered positive when specific O.D value was ≥0.5 at the 1:1000 dilution.
bImmune sera titers of BALB-neuT vaccinated mice were determined by ELISA against LTR-Neu and NIH3T3 using individual serum at different dilutions (1:1000, 1:2500, 1:12500). Sera titer represents the mean value of individual serum titer ± standard deviation (SD).
cTiter was estimated as the highest immune serum dilution generating a specific absorbance of 0.5 at 492 nm.
d108 pfu vs 107 pfu.
e108 pfu vs 106 pfu.