Figure 5. Cell adhesion to fibronectin is decreased with 50 µM 4N1K treatment.

Jurkat 6A and A1 cells were labeled with Celltracker Green, then treated with buffer, 50 µM 4NGG, 50 µM 4N1K, or 1 mM MnCl₂ for 20 min prior to seeding onto fibronectin-coated wells. In-well fluorescence was measured using a spectrofluorometer both before and after washes to remove non-adherent cells, and % adhesion was calculated as stated in methods and materials. Data as plotted are the means ± standard deviation for three replicate samples of one experiment (*, p<0.001).