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. 2014 May 12;5:19. doi: 10.1186/2041-9139-5-19

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Copyright © 2014 Glazer et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Marine/freshwater differences in gill raker number and spacing are specified early in development. (A-C) Ventral row 1 developing gill raker buds, stained for cartilage (Alcian blue) and bone (Alizarin red). Scale bar = 25 um. Fish total length is indicated in bottom left. (D, E) Time course of lab-reared mean row 1 ventral raker number (D) or left side row 1 ventral raker spacing (E)vs. total length of fish. Red = Little Campbell marine (LITC), light blue = Paxton benthic freshwater (PAXB), dark blue = Fishtrap Creek freshwater (FTC).