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. 2014 May 21;9(5):e98304. doi: 10.1371/journal.pone.0098304

Figure 2. Rad51 stimulates expression of integrated copies of HIV-1.

Figure 2

(A) TZM-bl cells multiple endogenous integrated copies of LTR-luciferase were transfected with 0, 3, 10 and 30 ng of pCMV (lanes 1–4 or 5–8, respectively). After 24 hours, cells either remained unchanged or infected with HIV-1 and 48 hours later cells were harvested for luciferase assay for promoter activity and Western blot for measuring Rad51 level. (B) TZM-bl cells were treated as above except that plasmid expressing siRad51 was used to decrease expression of endogenous Rad51. Luciferase activity and Rad51 Western blots are shown. (C) Primary human fetal astrocytes were transfected with luciferase reporter plasmid treated with RI-1 or B02 in various concentrations after 24 hours and luciferase activity was determined after 48 hours. (D) MTT assay showing cell viability upon treatment under the same conditions used in Panel C.