Figure 5. Inhibition of Rad51 function represses HIV-1 infection.

(A) Top: PBMCs isolated from 3 different donors were infected with HIV-1 SF₁₆₂ and incubated in the presence of 10 or 50 µM of Rad51 inhibitor (RI-1) for 3 days. Infection was quantified by examining the level of virus in the supernatants from the infected cells using p24 Gag ELISA. (B) Western blot analysis of protein extracts from the infected cells using anti-Rad51 and anti-α-tubulin antibody. The ratio of the intensity of the Rad51 and α-tubulin in each sample is shown. (C) MTT assay showing cell viability upon treatment under the same conditions used in Panel A.