Table 1.

Sequences of the oligonucleotide primers used in this study

Pathogen	Target gene	Oligonucleotide sequences (5′-3′)		Final amplicon size (bp)	Reference
		Forward	Reverse
Hemoparasite	18S rRNA	AACCTGGTTGATCCTGCCAGT	GATCCTTCTGCAGGTTCACCTAC	1750	Medlin et al., [20]
Theileria spp.	18S rRNA	AGTTTCTGACCTATCAG	TTGCCTTAAACTTCCTTG	1098	Allosop et al., [21]
T. annulata	30 kDa	GTAACCTTTAAAAACGT	GTTACGAACATGGGTTT	721	d’Oliveria et al., [22]
T. sergenti	MPSP	CACGCTATGTTGTCCAAGAG	TGTGAGACTCAATGCGCCTA	875	Liu et al., [23]
T. sinensis	MPSP	CACTGCTATGTTGTCCAAGAGATATT	AATGCGCCTAAAGATAGTAGAAAAC	887	Liu et al., [23]
T. orientalis	MPSP	CTTTGCCTAGGATACTTCCT	ACGGCAAGTGGTGAGAACT	776	Ota et al., [24]
T. cervi (Type F)	18S rRNA	TTCCCTTTGAGGGGT	AAGCCTATTCCCGTACC	654	Boes et al., [25]
T. cervi (Type G)	18S rRNA	CTTCCCGTTATGGAGG	CTTAACCTATTCCCGTGAG	654	Boes et al., [25]
T. luwenshuni	18S rRNA	GGTAGGGTATTGGCCTACTGA	TCATCCGGATAATACAAGT	340	Yin et al., [26]
T. uilenbergi	18S rRNA	GGTAGGGTATTGGCCTACCGG	ACACTCGGA AAATGCAAGCA	340	Yin et al., [26]
T. ovis	18S rRNA	TCGA-GACCTTCGGGT	TCCGGACATTGTAAAACAAA	520	Altay et al., [27]
T. lestoquardi	18S rRNA	GTGCCGCAAGTGAGTCA	GGACTGATGAGAAGACGATGAG	785	Aktas et al., [28]
T. capreoli	18S rRNA	gtcttgtaattggaatgatgg	ccaaagactttgatttctctc	580	Slemenda et al., [29]
Babesia spp.	18S rRNA	TGTCTTGAATACTT(C/G)AGCATGGAA	CGACTTCTCCTTTAAGTGATAAC	950	Ramos et al., [10]
B. odocoilei	18S rRNA	CACCGTATTTTGACTTTTTGTCGACTGTCGG	CCCGTAACGGACGAACCTTCTCACGGG	900	Ramos et al., [10]
B. bovis	18S rRNA	CTGTCGTACCGTTGGTTGAC	CGCACGGACGGAGACCGA	541	Guido et al., [30]
B. ovata	AMA-1	GATACGAGGCTGTCGGTAGC	AGTATAGGTGAGCATCAGTG	504	Sivakumar et al., [31]
B. bigemina	18S rRNA	TGGCGGCGTTTATTAGTTCG	CCACGCTTGAAGCACAGGA	1124	Guido et al., [30]
B. ovis	18S rRNA	TGGGCAGGACCTTGGTTCTTCT	CCGCGTAGCGCCGGCTAAATA	549	Aktas et al., [32]
B. motasi	18S rRNA	AAGAATTTCACCTCTGACAG	GCTTGCTTTTTGTTACTTTG	179	Shayan et al., [33]