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. 2014 May 21;9(5):e98380. doi: 10.1371/journal.pone.0098380

Figure 6. AAT mRNA and protein levels are increased in isolated proximal tubules harvested 18- induced AKI.

Figure 6

To demonstrate that the renal cortical AAT changes were reflective of proximal tubule events, proximal tubules were isolated from either normal mice or mice with glycerol induced AKI. AAT mRNA levels were markedly increased in post glycerol exposed tubules, compared to values observed in control tubules (left panel). For comparison, heme oxygenase 1 (HO-1) mRNA, known to be markedly induced in the glycerol model, was also measured and the degree of increase was less than that seen for AAT mRNA. As shown in the right hand panel, a 20 fold increase in AAT protein levels was observed in post glycerol exposed tubules. In sum, these proximal tubule AAT mRNA and protein levels indicate that the renal cortical AAT mRNA and protein increases reflected, at least in part, proximal tubule events.