Table 1. Primers used to measure mouse AAT and GAPDH mRNAs.
mRNA | Primer Sequences | Product Size |
Mouse AAT | 5′-TTC CAA CAC CTC CTC CAA AC -3′ 5′-CAC CGC CTC AGC TAT CTT TC-3′ | 234 bp |
Mouse Neutrophil Elastase | 5′-ACT GTG TGA ACG GCC TAA AT -3′ 5′-CTG GGT GAT GGT CTG TTT GT -3′ | 292 bp |
Mouse GAPDH | 5′-CTG CCA TTT GCA GTG GCA AAG TGG-3′ 5′-TTG TCA TGG ATG ACC TTG GCC AGG-3′ | 437 bp |
Mouse AAT and NE mRNAs were measured by RT-PCR and factored by simultaneously obtained GAPDH product.