Skip to main content
. 2014 May 21;9(5):e98380. doi: 10.1371/journal.pone.0098380

Table 1. Primers used to measure mouse AAT and GAPDH mRNAs.

mRNA Primer Sequences Product Size
Mouse AAT 5′-TTC CAA CAC CTC CTC CAA AC -3′ 5′-CAC CGC CTC AGC TAT CTT TC-3′ 234 bp
Mouse Neutrophil Elastase 5′-ACT GTG TGA ACG GCC TAA AT -3′ 5′-CTG GGT GAT GGT CTG TTT GT -3′ 292 bp
Mouse GAPDH 5′-CTG CCA TTT GCA GTG GCA AAG TGG-3′ 5′-TTG TCA TGG ATG ACC TTG GCC AGG-3′ 437 bp

Mouse AAT and NE mRNAs were measured by RT-PCR and factored by simultaneously obtained GAPDH product.