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. 2014 May 14;2:23. doi: 10.3389/fchem.2014.00023

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Copyright © 2014 Tang and Olsen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the chain extension products by SDS-PAGE. Lane 1: thiol-maleimide with 1a; lane 2: thiol-maleimide with 1b; lane 3: thiol-dibromomaleimide; lane 4: reduction of chain-extended proteins by BME; lane 5: thiol-ene. The abundance of high molecular weight species in thiol-ene and thiol-maleimide corresponds to their higher entanglement plateau.