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. 2014 May 15;8:174. doi: 10.3389/fnbeh.2014.00174

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Copyright © 2014 Vasmer, Pooryasin, Riemensperger and Fiala.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Temperature increase during behavioral experiments does not rapidly affect the expression of mCherry-dTRPA1 in Kenyon cells. Flies expressing mCherry-dTRPA1 in random subset of Kenyon cells were either raised and maintained at 18°C or raised at 18°C and transiently incubated for 30 min at 30°C at an age of 4–6 days. The duration of the elevated temperature matches the maximum time that animals can spend at temperatures above 25°C during all behavioral experiments. Subsequently the brains were dissected and the numbers of mCherry-dTRPA1 expressing Kenyon cells determined. Flies incubated at 18°C showed mCherry-dTRPA1 expression in 56.9 ± 8.1 (mean ± SD) Kenyon cells, whereas flies exposed for 30 min to 30°C showed expression in 48.0 ± 16.8 (mean ± SD) Kenyon cells (p > 0.2, Mann-Whitney-U-Test).