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. 2014 May 14;5:187. doi: 10.3389/fpls.2014.00187

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Copyright © 2014 Kleist, Spencley and Luan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Close-up of Type III CBL clade from ML tree shown in Figure 2. Confidence scores (aLRT) are shown for each clade. Arabidopsis CBL10 (green dot) and orthologs in angiosperms and gymnosperms that share the presence of a single N-terminal transmembrane helix (see Figure 3) form a well-supported clade (aLRT = 0.96). Physcomitrella CBL4, which also appears to contain a N-terminal transmembrane helix, does not phylogenetically cluster with this clade or other angiosperm homologs, possibly due to sparse taxon sampling among bryophytes.