Figure 8.

ML phylogenetic tree derived from protein MSA of all CIPKs identified in this study. Confidence scores (aLRT) are shown for select clades. CIPKs from green algae phylogenetically cluster with land plant CIPKs, including Physcomitrella CIPK6 and CIPK7. Remaining Physcomitrella CIPKs cluster with Arabidopsis CIPK1, 17, and 21, which contain multiple introns, and a clade of “intronless” CIPKs (although AtCIPK16 has gained one intron) derived from an inferred reverse transcription event (yellow star). See Supplementary Table S3 for CIPKs in this study, Supplementary File S3 for MSA, and Supplementary File S4 for full phylogenetic tree with tip labels. (^*Although AtCIPK16 has gained one intron.)