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. 2014 May 14;5:195. doi: 10.3389/fpls.2014.00195

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Copyright © 2014 Lin, Pierce, Fang, Wise, Binns and Lynn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chimeric GCN4 fusions with 450^K and 450^KR. (A) Design of the GCN4-450 fusions. The heptad repeats from a to g were built from the registry of GCN4 and the adapters. GCN4 enforces the hydrophobic ad interface (shown in bold), and shifts the registry of the heptads of kinase coiled-coil according to the different adapters. The predicted position of His474 (^*in the K domain) in each fusion is shown at the end of the sequence. (B) A. tumefaciens strain A136 carrying pRG109 and the indicated GCN4-450^KR fusions were assayed for vir gene expression without inducers. The degree of rotation created by each fusion is shown in the figure with the 0° rotation defined at LZ(0)-450. The protein expression of the GCN4-450^KR constructs was analyzed by Western blot.