Figure 4.

Direct amino acid insertion within the coiled-coil. (A) The amino acids in bold were inserted between amino acids 449 and 450 in the predicted coiled-coil region. (B) An illustration of how His474 moves along the helix coil according to the amino acid insertion at the N′-terminus. (C) A. tumefaciens strain A136 carrying pRG109 and the LK constructs with different insertions at aa449 (449⁺ⁿ) were assayed for vir gene expression with or without 300 μM AS. The degree of rotation created by the insertions is shown in the graph.