Figure 6.
Library screen for constitutive mutations within α4 (aa426–437). (A) β-galactosidase activity of the identified constitutively induced mutants. A. tumefaciens strain A136 carrying pRG109 and wild-type LKR or LKR mutants were assayed for vir gene expression with or without 300 μM AS. (B) A. tumefaciens strain A136 carrying pRG109 and wild-type 426K or 426K mutants were assayed for vir gene expression without inducers.