Table 2.
Most closely related (based on BLASTn-determined similarity) 16S rRNA gene sequences from environmental surveys and cultured microorganisms to OTU0.03 comprising ≥0.5% of sequences in libraries recovered from different depth in the MF iron mound.
| OTU # | Nearest environmental 16S rRNA gene sequence | Nearest cultured 16S rRNA gene sequence |
|---|---|---|
| 1 | AMD-impacted Río Tinto (99%; FN867143; Amaral-Zettler et al., 2011) | Spirochaeta aurantia from freshwater (94%; AJ565432; Hahn et al., 2004) |
| 2 | AMD-impacted Río Tinto (98%; FN863828; Amaral-Zettler et al., 2011) | None found |
| 3 | “Iron snow” in acidic mine lake (99%; HE604017; Lu et al., 2013) | Steroidobacter denitrificans FS, NO3− reducer from anoxic sludge (94%; NR_044309) (Fahrbach et al., 2008) |
| 4 | Abandoned Cu mine (99%; JQ217995; Falteisek and Cepička, 2012) | Halothiobacillus kellyi BII-1, thermophilic S oxidizer (84%; NR_025030; Sievert et al., 2000) |
| 5 | “Iron snow” in acidic mine lake (99%; HE604030; Lu et al., 2013) | Acidophilic FeOB C4H7 (99%; JX869450; Wu et al., 2013) |
| 6 | Abandoned Cu mine (99%; JQ217802; Falteisek and Cepička, 2012) | Metallibacterium X11 adidophilic S2O32- oxidizer (95%; HE858262; Delavat et al., 2012) |
| 7 | AMD-impacted Río Tinto (96%; FN866063; Amaral-Zettler et al., 2011) | None found |
| 8 | Arctic stream epilithon (97% FJ849138; Larouche et al., 2012) | Elphidium aculeatum A75.46 chloroplast (85%; HM213365; Pillet et al., 2011) |
| 9 | Abandoned Cu mine (99%; JQ217544; Falteisek and Cepička, 2012); AMD iron mound (99%; HQ420151; Brown et al., 2011) | Ferrovum myxofaciens EHS8, acidophilic FeOB (97%; KC155322; Hedrich et al., 2009); Ferrovum myxofaciens PSTR, acidophilic FeOB (97%; EF133508; Hallberg et al., 2006) |
| 10 | AMD-impacted sediment (99%; EF409850; Yin et al., 2008) | Acidophilic FeOB/FeRB iFeo-D4-31-CH (94%; FN870336; Lu et al., 2010) |
| 11 | AMD-impacted Río Tinto (99%; FN862195; Amaral-Zettler et al., 2011) | Euglena mutabilis SAG 1224-9b chloroplast (96%; AY626044; Milanowski et al., 2006) |
| 12 | Metal sulfide mine AMD (99%; GU979565; Hao et al., 2010) | Rhizobiales strain A48, neutrophilic FeRB (94%; AB081581; Satoh et al., 2002) |
| 13 | AMD-impacted Río Tinto sediment (99%; JF737887; García-Moyano et al., 2012) | Aciditerrimonas ferrireducens, thermoacidophilic FeRB (92%; AB517669; Itoh et al., 2011) |
| 14 | Acidic mine lake (99%; KC619609; Santofimia et al., 2013) | Acidophilic heterotrophic FeOB Py-F3 (96%; KC208497; Kay et al., 2013) |
| 15 | Acidic wetland soil (99%; GQ203360; Kopecky et al., 2011) | Actinoallomurus sp. 645152, acidophilic organotroph (96%; AB604840; Murmatsu et al., 2011) |
| 16 | Acidic mine lake sediments (99%; FN870199; Lu et al., 2010) | Acidophilic FeOB A4F6 (93%; JX869415; Wu et al., 2013) |
| 17 | AMD iron mound (97%; HQ420151; Brown et al., 2011); AMD-impacted Río Tinto (97%; FN867145; Amaral-Zettler et al., 2011) | Ferrovum myxofaciens EHS8, acidophilic FeOB (97%; KC155322; Hedrich et al., 2009); Ferrovum myxofaciens PSTR, acidophilic FeOB (97%; EF133508; Hallberg et al., 2006) |
| 18 | “Iron snow” in acidic mine lake (99%; HE604029; Lu et al., 2013) | Arhodomonas sp. Seminole, aerobic halophile (84%; JX099567; Dalvi et al., 2012) |
| 19 | Abandoned Cu mine (99%; JQ217975; Falteisek and Cepička, 2012) | Leptothrix ochracea SCGC AAA018-M4 FeOB (98%; HQ290506; Fleming et al., 2011) |
| 20 | AMD-impacted Río Tinto (99%; FN860398; Amaral-Zettler et al., 2011) | Acidophilic FeOB A4F5 (98%; JX869415; Wu et al., 2013) |
| 21 | Acidic hot spring (97%; JF280561; Bohorquez et al., 2012) | Anaerobic bacterium BSV83 (86%; AJ229227; Hengstmann et al., 1999) |
| 22 | AMD-impacted Río Tinto (99%; FN866617; Amaral-Zettler et al., 2011) | Lysobacter sp. AP7 (82%; EU374884; Alonso-Gutiérrez et al., 2009) |
| 23 | Abandoned Cu mine (99%; JQ218102; Falteisek and Cepička, 2012) | Acidophilic, organotrophic Acidobacteriaceae CH1 from AMD (97%; DQ355184; Diaby et al., 2007) |
| 24 | AMD-impacted Río Tinto sediment (99%, HQ730615; Sánchez-Andrea et al., 2011) | Acidophilic FeOB A4F5 (98%; JX869414; Wu et al., 2013) |
| 25 | Abandoned Cu mine (99%; JQ217544.1; Falteisek and Cepička, 2012) | Ferrovum myxofaciens EHS8, acidophilic FeOB (99%; KC155322; Hedrich et al., 2009); Ferrovum myxofaciens PSTR, acidophilic FeOB (99%; EF133508; Hallberg et al., 2006) |
| 26 | AMD biofilm (99%; JX297618.1; Guo et al., 2013) | Metallibacterium X11 adidophilic S2O32− oxidizer (99%; HE858262; Delavat et al., 2012); Metallibacterium scheffleri DKE6, acidophilic facultative FeRB (98%; HQ909259.1; Ziegler et al., 2013) |
| 27 | AMD-impacted Río Tinto (99%; FN862147; Amaral-Zettler et al., 2011) | Thiohalophilus thiocyanatoxydans HRhD 2, halophilic S oxidizer (91%; NR_043875; Sorokin et al., 2006) |
| 28 | Volcanic deposits (88%; AY917857; Gomez-Alvarez et al., 2007) | Thermogemmatispora onikobensis, thermophilic organotroph (86%; AB547912; Yabe et al., 2011) |
| 29 | Abandoned Cu mine (100%; JQ217580; Falteisek and Cepička, 2012) | Metallibacterium X11 adidophilic S2O32− oxidizer (100%; HE858262; Delavat et al., 2012); Acidophilic Fe(II) oxidizer C4C8 (97%; JX869446; Wu et al., 2013); Metallibacterium scheffleri DKE6, acidophilic facultative FeRB (97%; HQ909259.1; Ziegler et al., 2013) |
| 30 | “Iron snow” in acidic mine lake (99%; HE604014; Lu et al., 2013) | Acidophilic FeOB A10G4 (99%; JX869422; Wu et al., 2013) |
| 31 | Reject coal-impacted soil (97%; AF523920; Brofft et al., 2002) | Moorella sp. 64_FGQ, thermophlilic FeRB (87%; GQ872425; Nepomnyashchaya et al., 2012) |
| 32 | Metal sulfide mine AMD (99%; GU979565; Hao et al., 2010) | Alicyclobacillus acidoterrestris C-ZJB-12-17, thermoacidophilic (96%; KC193190; Zhang et al., 2013) |
| 33 | Volcanic deposits (90%; AY425781; Gomez-Alvarez et al., 2007) | Thermogemmatispora onikobensis, thermophilic organotroph (85%; AB547912; Yabe et al., 2011) |
| 34 | AMD-impacted Río Tinto (99%; FN863733; Amaral-Zettler et al., 2011) | Leptospirillum ferrooxidans C2-3 acidophilic FeOB (99%; NR_074963) Fujimura et al., 2012 |
| 35 | AMD-impacted Río Tinto sediment (100%; JF737887; García-Moyano et al., 2012) | Chloroflexi SCGC AAA007-G23, marine S oxidizer (82%; HQ675468; Swan et al., 2011) |
| 36 | AMD-impacted creek (99%; HE653802; Volant et al., 2012) | Methanomassiliicoccus luminyensis B10, methanogen (85%; HQ896499; Dridi et al., 2012) |
| 37 | Anoxic rice field soil (89%; FM956256; Gan et al., 2012) | Thermoactinomyces vulgaris 55N1-5, thermophilic organotroph (88%; JN366723; Lima et al., 2012) |
| 38 | AMD-impacted Río Tinto (99%; FN862217; Amaral-Zettler et al., 2011) | Acidithiobacillus ferrivorans SS3, FeOB/facultative FeRB (99%; NR_074660; Liljeqvist et al., 2011) |
| 39 | Abandoned Cu mine (99%; JQ218054; Falteisek and Cepička, 2012) | Clostridium ghonii 2447_6, fermentative anaerobe (76%; JN048963; Ryzinska-Paier et al., 2011) |
| 40 | Deep granitic fracture water (88%; 7150D1B75; Sahl et al., 2008) | Pelotomaculum terephthalicicum JT strain J, syntroph (85%; NR_040948; Qiu et al., 2004) |
| 41 | AMD biofilm (95%; JX297618.1; Guo et al., 2013) | Metallibacterium X11 adidophilic S2O32− oxidizer (94%; HE858262; Delavat et al., 2012); Acidophilic FeOB C4C8 (94%; JX869446; Wu et al., 2013); Metallibacterium scheffleri DKE6, acidophilic facultative FeRB (94%; HQ909259.1; Ziegler et al., 2013) |
| 42 | Alpine tundra soil (94%; FJ570063; Zinger et al., 2009) | Myxobacterium sp. KC, humic substance oxidizer (93%; AF482687; Coates et al., 2002) |
| 43 | AMD-impacted creek (99%; HE653802; Volant et al., 2012) | Methanomassiliicoccus luminyensis B10, methanogen (85%; HQ896499; Dridi et al., 2012) |
| 44 | Anoxic rice field soil (87%; FM956256; Gan et al., 2012) | Thermoactinomyces vulgaris 55N1-5, thermophilic organotroph (86%; JN366723; Lima et al., 2012) |
| 45 | Acidic mine lake (93%; KC619609; Santofimia et al., 2013) | Desulfosporosinus sp. GBSRB4.2 (99%; EU839714; Senko et al., 2009) |
| 46 | Arctic fell-field soil (94%; EF221592; Yergeau et al., 2007) | Ktedobacter racemifer SOSP1-21, aerobic organotroph (88%; AM180160; Cavaletti et al., 2006) |
| 47 | AMD-impacted Río tinto (97%; FN860399; Amaral-Zettler et al., 2011) | Chloroflexi SCGC AAA007-G23, marine S oxidizer (79%; HQ675468; Swan et al., 2011) |
| 48 | AMD-impacted Río Tinto (99%; FN860359; Amaral-Zettler et al., 2011) | Lysobacter sp. AP7 (80%; EU374884; Alonso-Gutiérrez et al., 2009) |
| 49 | Acidic bog (99%; JQ807564; Lin et al., 2012) | Crenarchaeote OS70 (87%; EU239962; De la Torre et al., 2008) |
OTU numbers in the left hand column refer to OTU number designations in Figure 5. Percent similarities, GenBank accession numbers, and appropriate references of sequences are provided in parentheses.