Table 1.
Primers and conditions for reverse-transcription PCR.
| Subunit | GenBank Acc. No. | Primer Sequence (5’-3’);Tm [°C] | Tann[°C] | amplicon [bp] | Ref |
|---|---|---|---|---|---|
| α1 | NM_001127644.1 | F: TGAGCACACTGACTGGAAGAAGC; 62,4 | 55 | 999 | Maddox et al. (2004) |
| R: GAACCACACTTTTGCCATCCC; 59,8 | |||||
| α6 | NM_000811.2 | F: TGATGGTCAGTAAAATCTGGACGC; 61,0 | 55 | 507 | Maddox et al. (2004) |
| R: AAACAGTTCTTGCTGGGACGG-3’; 59,8 | |||||
| β2 | NM_021911.2 | F: TGCCTGATACCTATTTCCTGAACG-3’; 61,0 | 55 | 488 | Maddox et al. (2004) |
| R: GATTCCTAATGCCACCCTTGC-3’; 59,8 | |||||
| Δ | NM_000815.4 | F: AGGACATCGTCTACTACTGGTCGGAGAG-3’; 64,4 | 55 | 439 | Maddox et al. (2004) |
| R: TCGGCGTTGAAATGAGCAAAGG-3’; 60,3 | |||||
| γ 2 | NM_198904.2 | F: TGCACACTCATTGTCGTCCTATCCTGG-3’; 66,5 | 60 | 524 | Maddox et al. (2004) |
| R: TTAAACAGGCAGAAGGCAGTGGGG-3’; 64,4 | |||||
| hGAPDH | NM_017008 | F: cacccatggcaaattccatg; 57.3 | 55 | 372 | Einarsen et al. (2009) |
| R: catgagtccttccacgatac; 57.3 |
Forward (F) and reverse (R) primers used for RT-PCR of human GABAA subunits. Expected amplicon sizes refer to cDNA according to GenBank accession numbers.