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. 2014 May 15;4:64. doi: 10.3389/fcimb.2014.00064

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Copyright © 2014 Park, Allen, Barrington and Davis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Map culture results from nine tissue sites processed at necropsy. The data are expressed as the average CFU of triplicate agar cultures containing no hygromycin. No colonies were detected in the agar cultures containing hygromycin, which indicates ΔrelA was cleared in the vaccinated animal. JP, JM, JD: jejunum proximal, middle, distal; IP, IM, ID: ileum proximal, middle, distal; ICV: ileocecal valve; ML, IL: mesenteric and ileocecal lymph nodes. a, Negative; b, too numerous to count; c, Values are expressed as the average CFU obtained from three agar cultures; +, positive by IS900 qPCR.