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. 2014 May 15;4:64. doi: 10.3389/fcimb.2014.00064

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Copyright © 2014 Park, Allen, Barrington and Davis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gating strategy used to analyze the proliferative response of peripheral blood mononuclear cells (PBMC) ex vivo following stimulation with live Map. Representative CD4 profiles obtained with PBMC from a calf inoculated/vaccinated with ΔrelA and challenged with wild type Map. (A) Selective gates were placed on resting/unstimulated cells (G1) and stimulated proliferating cells (G2) and color coded to track activated cells. (B) Cells with no mAb. (C) Cells labeled with CD4 with no gate to show relative percent of resting and activated CD4 cells. (D) CD4 cells with a gate to select CD4 cells for analysis. (E) CD4 cells showing expression of CD45R0 only on memory cells. The activated cells (blue) also expressed CD25.