Figure 7.

Relative transcription of genes involved in Th1 type immune response in PBMC. PBMC were isolated from animals in each group and cultured with and without live Map for 3 days. The level of mRNA transcription of PBMC was measured by qRT-PCR. The relative quantification of each cytokine was calculated using the value of PBMC isolated from control animals (uninfected animals) and cultured in RPMI medium alone as the calibrator. The panels show the relative quantification of IL-12p35 (A), Tbet (B), IFN-γ (C). RQ, relative quantification; RPMI and MAP, PBMC cultured in RPMI medium alone and with live Map, respectively; ^* and ⋆, significant difference compared to RPMI and MAP of the control group, respectively.