Figure 4.

(a) SSCP profiles of partial 16S rRNA gene products PCR amplified from density gradient fractions of soil DNA from microcosms incubated with ¹³C-phenol. Comparisons of results obtained from soil variants NIL, NPK and FYM, incubated over a period of 4 days (left panel) and 7 days (right panel), respectively. Brackets indicate lanes with density fractions in the range of 1.71–1.73 g ml⁻¹. Each band represents a specific PCR product. No specific PCR products were detected in the corresponding density fractions with DNA from soil incubated with unlabeled (¹²C) phenol as a control (shown on SSCP gels in the Supplementary Figure S1). For more details of the DNA sequences from these bands, see Supplementary Figure S3 and Supplementary Table S3. (b). Phylogenetic assignment of partial 16S rRNA genes from clone libraries of the same soil DNA solutions after 4 days of incubation (for more details, see Supplementary Table S4).