Figure 5.

(a) SSCP profiles of PCR amplified fungal 18S-ITS1-5.8S rRNA gene products from density gradient fractions of soil DNA from microcosms incubated with ¹³C-phenol. Results are shown for samples obtained from soil variants NIL, NPK and FYM, incubated over a period of 4 days (left panel) and 7 days (right panel), respectively. Brackets indicate lanes with density fractions in the range of 1.71–1.73 g ml⁻¹. Each band indicates a specific PCR product. No specific PCR products were detected in the corresponding fractions with DNA from control soil incubated with unlabeled phenol (see Supplementary Figure S2). More details on the DNA sequences from the bands detected from the fractions with ¹³C-enriched DNA are shown in the Supplementary Figure S4 and Supplementary Table S5. (b) Phylogenetic assignment of the 18S-ITS1-5.85S-ITS2-28S rRNA sequences from clone libraries of the same soil DNA solutions after 4 days of incubation (for more details, see Supplementary Table S6).