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. 2014 Jan 16;8(6):1289–1300. doi: 10.1038/ismej.2013.233

Figure 6.

Figure 6

SSCP profiles of partial 16S rRNA gene products PCR amplified from density gradient fractions of soil DNA from microcosms incubated with 13C-DCP. Comparisons of results obtained from soil variants NIL, NPK and FYM, incubated over a period of 9, 14 and 21 days, respectively. Brackets indicate lanes with density fractions in the range of 1.71–1.73 g ml−1. Each band represents a specific PCR product. No specific PCR products were detected in the corresponding density fractions with DNA from control soil incubated with unlabeled (12C) DCP (Supplementary Figure S5). Bands (DNA) indicated by numbers 1–6 were sequenced and characterized for the phylogenetic assignment (see Figure 7).