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. 2014 Apr 5;11:71. doi: 10.1186/1742-2094-11-71

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Copyright © 2014 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PD168393 inhibited epidermal growth factor receptor (EGFR) phosphorylation of astrocytes after spinal cord injury (SCI) in rats. Spinal cord sections were immunostained for phosphorylated EGFR (pEGFR) and glial fibrillary acid protein (GFAP) at two weeks after SCI (n = 5/group). (A1-C1) GFAP, (A2-C2) pEFGR, and (A3-C3) co-localization of pEGFR and GFAP. Insets in panels (B1-C3) are shown at high magnification in panels (D1-E3), respectively. Scale bars = 500 μm in (C3) (applies to A1-C3); scale bars = 50 μm in (E3) (applies to D1-E3). Representative Western blots of pEGFR and EGFR expression (n = 3/group), and β-actin was a loading control (F). Semi-quantitative measurements were obtained by normalizing to β-actin (G). *P < 0.05.