Figure 2.

Viral constructs knocked down disrupted in schizophrenia 1 (DISC1) expression in rat prefrontal cortex (PFC). (a) Western blots verified that the Disc1–short hairpin-expressing RNA1 (shRNA1) and Disc1–shRNA2 constructs effectively knocked down expression of full-length rat DISC1 in HEK293 cells in two separate trials (Experiments 1 and 2). Disc1–shRNA1 was subsequently used in the behavioral experiments. (b) Rats received viral infusions in PFC (AP −3.2 mm; ML±0.75 mm; DV −4.2 mm) (1) or dorsal to PFC in cingulate cortex area 1 (Cg1)/secondary motor cortex (M2) (Cg1/M2; AP −3.2 mm; ML±0.75 mm; DV −2.0 mm) (2) (left, middle), and DISC1 labeling in these regions was compared with that in primary motor cortex (M1). Shaded areas indicate regions compared for stereology (right). Small rectangles represent regions compared for optical densitometry and % area of DISC1 labeling (right); actual sites and orientation varied from field to field.