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. 2013 Dec 3;3(12):e327. doi: 10.1038/tp.2013.99

Figure 6.

Figure 6

14–3-3ζ Regulates dopamine transporter (DAT )expression in the substantia nigra (SN)-ventral tegmental area (VTA). (a) Sagittal brain sections stained with anti-tyrosine hydroxylase (TH; red) and anti-DAT (green) show reduced levels of DAT in the SN-VTA of 14-3-3ζ knockout (KO) mice compared with wild-type (WT) littermates. Blue, 4,6-diamidino-2-phenylindole (DAPI); scale bars=50 um. (b) Higher magnification of anti-DAT immunostaining (green) in SN-VTA shows that DAT is mislocalized in the cell bodies of dopaminergic neurons (white arrowheads). Scale bar=20 um. (c) Quantitation of anti-DAT immunostaining normalized to anti-TH confirms that 14-3-3ζ KO (open bar) have an approximately 30% reduction of DAT compared with WT mice (closed bar).