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. 2013 Dec 3;3(12):e327. doi: 10.1038/tp.2013.99

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Copyright © 2013 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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14–3-3ζ Associates with dopamine transporter (DAT). (a) SN4741 cells were transiently transfected with Flag-His-DAT and Myc-14-3-3ζ. 14-3-3ζ was precipitated from protein lysates with anti-Myc antibody. 14-3-3ζ Immunoprecipitates were probed with anti-Myc monoclonal antibody to recognise 14-3-3ζ and anti-Flag to recognize DAT. (b) DAT was precipitated from protein lysates as in (a) with anti-Flag antibody. DAT immunoprecipitates were probed with anti-Myc monoclonal antibody to recognise 14-3-3ζ and anti-Flag to recognize DAT (c) 14-3-3ζ Was precipitated from P35 striatum lysates with anti-14-3-3ζ G1-7 monoclonal antibody. 14-3-3ζ Immunoprecipitates were probed with anti-14-3-3ζ (C16) and antibodies against DAT.