Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Cell Biochem. 2014 Jan;115(1):71–80. doi: 10.1002/jcb.24634

Figure 2. Lithium pretreatment enhances TLR-2 and TLR3-induced TNFα production in mouse macrophages.

Figure 2

Raw264.7 cells were plated in 12-well plate as indicated in Figure 1. In A, Cells were pretreated with NaCl or LiCl (30 mM) for 6 hours and then treated with Pam3CSK4 (1 μg/ml) or Poly I:C (μg/ml) for the indicated time points. Culture supernatants were then assayed for TNFα using ELISA. In B, cells were treated with NaCl/LiCl for 6 hours with the various concentrations as shown and then treated with Pam3CSK4 (1 μg/ml) or Poly I:C (1 μg/ml). Supernatants were then assayed for TNFα using ELSIA.N=3-8 for A and N=6 for B. *P<0.05; **P<0.01; ***P<0.001; compared between the respective concentrations of LICL NaCl.