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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Cell Biochem. 2014 Jan;115(1):71–80. doi: 10.1002/jcb.24634

Figure 3. Lithium's effect on TNFα in mouse macrophages is at the post-transcriptional level and downstream of TLR expression.

Figure 3

(A) Raw264.7 cells were pretreated with NaCl or LiCl (10 mM) for 6 hours followed by treatment TLR ligands as indicated for additional 6 hours. Cells were then processed for RNA extraction and TNFα mRNA expression determined by real time QPCR as described before [Loniewski et al., 2007].(B) Raw264.7 macrophages were treated with NaCl or LiCl (30 mM) for 3 or 6 hours and the expression levels of the different TLRs examined by flow cytometry. A representative experiment from two such experiments is shown