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. Author manuscript; available in PMC: 2015 Jan 1.

Published in final edited form as: J Cell Biochem. 2014 Jan;115(1):71–80. doi: 10.1002/jcb.24634

Raw264.7 macrophages were plated as described in Fig 1 and then treated with NaCl or LiCl (30 mM) for 6 hours followed by treatment with Pam3CSK4 (1 μg/ml) for the indicated time points. Cells were then processed and Western blotting performed for the various proteins as shown (A and B). N=3. *P<0.05; **P<0.01; ***P<0.001; compared between NaCl and LiCl at the respective time points. In C, effect of IKKβ inhibitor (IKK-2 inhibitor IV, Calbiochem) on Pam3CSK4-induced pERK levels is shown. Cells were treated with NaCl or LiCl followed by IKKβ inhibitor prior to stimulation with Pam3CSK4. PhosphoERK levels were analyzed by Licor Odyss y as described in the methods. N=3.

Raw264.7 macrophages were plated as described in Fig 1 and then treated with NaCl or LiCl (30 mM) for 6 hours followed by treatment with Pam3CSK4 (1 μg/ml) for the indicated time points. Cells were then processed and Western blotting performed for the various proteins as shown (A and B). N=3. *P<0.05; **P<0.01; ***P<0.001; compared between NaCl and LiCl at the respective time points. In C, effect of IKKβ inhibitor (IKK-2 inhibitor IV, Calbiochem) on Pam3CSK4-induced pERK levels is shown. Cells were treated with NaCl or LiCl followed by IKKβ inhibitor prior to stimulation with Pam3CSK4. PhosphoERK levels were analyzed by Licor Odyss y as described in the methods. N=3.