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Colocalization of intracellular EGFR and CEA. HT-29 cells that express both EGFR and CEA show that agents targeted to these two receptors will colocalize to a considerable extent to the same intracellular compartments, a necessary condition for the success of the proposed potentiation strategy. An anti-EGFR antibody was conjugated with AlexaFluor-488 and an anti-CEA scFv was conjugated with AlexaFluor-594 before both were used to label HT-29 cells to observe colocalization.
