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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Bone Miner Metab. 2013 Mar 26;31(5):520–532. doi: 10.1007/s00774-013-0449-6

Fig. 7.

Fig. 7

β-catenin signaling in MC3T3-E1 cells was dependent on the existence of osteocytes under clinorotation. (A) MC3T3-E1 cells cocultured with MLO-A5 cells were transfected with a TCF/LEF reporter and subjected to clinorotation for 4 h. Scale bar 10µm. (B) The time course of TCF/LEF activity in MC3T3-E1 cells in the cocultures under clinorotation. *** P < 0.001. n = 7 cells. (C) Bar graphs represent changes in TCF/LEF activity of MC3T3-E1 cells under clinorotation in the two different culture conditions (monocultures versus cocultures) at three time points; 0, 2, and 4 h. Data were normalized to the basal TCF/LEF activity in monocultures at 0 h. *** P < 0.001. At least six cells were analyzed.