Figure 5. Relationship of DNAme Status to Histone Modifications across Development.
(A) Epigenetic features at TSS regions (±250 bp). Histone modifications (mean log 2 ratio, scale –1.5 to 1.5, array data: sperm [Wu et al., 2011a]; preZGA (2.5 hpf), ZGA (3.3 hpf), and postZGA (5.3 hpf) [Lindeman et al., 2011]). DNAme: mean faction CG methylation at sperm and sphere stage, with k-means clustering analysis. (B) Snapshots of the krt4 gene, as an example of a gene from cluster 7 whose expression is highly upregulated by 5-Aza-CyD treatment. DNAme (mean base fraction CG methylation, scale 0 to 1), gene expression (RPKM, scale 0–25, from RNA-seq of total RNA), histone modifications (probe log 2 ratio, scale 0–1.5 at postMBT; [Lindeman et al., 2011]) visualized on IGB.