Fig. 3.

A) Diagram representing devices shown in B and C. B) Diversion of flow through polymerization based amplification of the positive biotin/avidin recognition by the glucose oxidase-avidin complex. C) Lack of flow diversion when polymerization based amplification is performed on a system exposed to unconjugated glucose oxidase. D) Backpressure resulting from exposure to 0 or 10 μg mL⁻¹ TGF-β and subsequent polymerization based amplification of a device built to detect TGF-β. A syringe pump generates pressure by depressing an air-filled syringe at a rate of 50 μL s⁻¹.