FIG 5 .

H5N1-VN1203 mediates control over the global ISG response primarily through the activity of NS1. (A) RNA expression of downregulated ISGs following each infection condition. Downregulated gene list was derived from Fig. 1C, and values represent fold change (log₂ FC) compared to time-matched mocks. (B and C) ChIP against H3K4me3 (B) and H3K27me3 (C) followed by qPCR of the 5′UTR of the identified genes within the context of H5N1-VN1203 (blue) or H5N1 NS1trunc124 (white) infection. Values represent fold increases in binding compared to mock on a log₂ scale. (D) Global transcription of consensus ISG from A549 cells infected with WT 1918 H1N1 or 1918 H1N1 encoding TX/91 NS1 (MOI of 2). RNA expression values represent fold change (log₂ FC) compared to time-matched mock.