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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: CNS Neurol Disord Drug Targets. 2013 Sep;12(6):750–762. doi: 10.2174/18715273113126660171

Fig. (2).

Fig. (2)

Zinc finger nuclease (ZFN)-mediated DNA double-strand break. A ZFN designed to create a DNA double-strand break (DSB) in the target consists of two monomers. Each monomer encompasses three zinc-fingers (1, 2, 3), which recognize 9 base pairs within the target and a FokI nuclease domain. A short “linker” sequence connects the two domains. The FokI nuclease only functions as a dimer and therefore, following dimerization the nuclease is activated and cleaves the DNA within the spacer sequence.