Figure 1. Pre-miR-24-2 decreases PKCα and PKCα-mediated gene expression in vitro.
(A) MCF-7-vector and −pre-miR-24-2 cell lines were harvested for total RNA. qPCR was performed for PKCα. Cycle number was normalized to beta-actin and vector control designated as 1. (B) Cells were harvested for total protein extraction and western blot analysis was performed for total PKCα expression. Loading control was alpha tubulin, n=1. (C) qPCR was performed for MMP-1 following 24 hours of treatment with 20ng/ml PMA or vehicle control. Cycle number was normalized to beta-actin and vector designated as 1, results represent relative expression value. *** Significantly different from DMSO treated vector, p < 0.001. Error bars represent SEM, n > 3 independent biological repeats.