Figure 2.

Growth of MCF7 breast cancer epithelial cells, but not non-transformed ‘normal’ HB2 breast epithelial cells, was stimulated by immortalized breast fibroblasts. GFP-labelled MCF7 breast cancer cells (A) or HB2 benign breast epithelial cells (B) were co-cultured with immortalized breast fibroblasts (pictured), or were cultured alone, and epithelial cell growth was monitored by counting GFP-positive cells using flow cytometry for up to 10 days. Data are means of biological triplicates (± standard error) and are representative of duplicate experiments.