Figure 3.

MiR-26b expression was frequently down-regulated in CAFs compared with matched NFs. (A) Samples enriched for fibroblasts were isolated by LMD from samples of matched breast cancer and normal tissue from 14 sequential cases of luminal A breast cancer (see Supplementary Table 1). (B) Primary cultures of matched NFs and CAFs were established from four further breast cancer cases. qPCR was used to analyse miR-26b expression relative to the geometric mean RNU6B and RNU48. Data are means of technical triplicates (± standard error).