Skip to main content
. 2014 May 15;171(9):770–778. doi: 10.1016/j.jplph.2013.09.014

Fig. 4.

Fig. 4

Chloride contents and analysis of Cl fluxes at the plasma membrane and tonoplast. Outputs resolved over a standard diurnal cycle (12 h light:12 h dark, indicated by bars above) (Chen et al., 2012) for the wild-type (left) and the ost2 mutant (right). Shown are (A) total cytosolic and vacuolar [Cl], (B) the net flux of Cl across the plasma membrane and tonoplast, and (C) the flux of Cl through the Cl-permeable transporters at the plasma membrane (SLAC and R- (ALMT-) type anion channels and H+–Cl symporter). Again, positive flux is defined as movement of the ionic species (not the charge) out of the cytosol, either across the plasma membrane or the tonoplast. Note the elevation in [Cl] in both the cytosol and vacuole, and the loss in their dynamics in the ost2 mutant. Closure was marked by a large flux of Cl from the vacuole to the cytosol and export across the plasma membrane, but the pattern was lost in the mutant, reflecting the loss of cyclic changes in voltage and [Ca2+]i that drive solute efflux from the vacuole and across the plasma membrane [see Chen et al. (2012)].