Table 2.
Kinetic Properties of SHV-1 and Asn276Asp for Ampicillin, Piperacillin, Nitrocefin, and Cephalothin
| SHV-1 | Asn276Asp | |
|---|---|---|
| Ampicillin | ||
| Km(μM) | 183 ± 34 | 134 ± 13 |
| kcat (s−1) | 3100 ± 300 | 989 ± 99 |
| kcat/Km (μM−1 s−1) | 17 ± 4 | 7 ± 1 |
| Piperacillin | ||
| Km(μM) | 77 ± 7 | 105 ± 18 |
| kcat (s−1) | 898 ± 90 | 976 ± 98 |
| kcat/Km (μM−1 s−1) | 12 ± 1 | 9 ± 1 |
| Nitrocefin | ||
| Km(μM) | 21 ± 3 | 28 ± 3 |
| kcat (s−1) | 237 ± 24 | 137 ± 14 |
| kcat/KM (μM−1 s−1) | 11 ± 2 | 5 ± 1 |
| Cephalothin | ||
| Km(μM) | 29 ± 1 | 83 ± 11 |
| kcat (s−1) | 14 ± 1 | 10 ± 1 |
| kcat/Km (μM−1 s−1) | 0.47 ± 0.03 | 0.12 ± 0.02 |