Skip to main content
. Author manuscript; available in PMC: 2014 May 22.
Published in final edited form as: Biochemistry. 2009 Jun 2;48(21):4557–4566. doi: 10.1021/bi9003292

Table 3.

Kinetic Properties of SHV-1 and Asn276Asp for Clavulanate, Cephalothin BATSIs, Methylidene Penem, and Meropenem

SHV-1 Asn276Asp
Clavulanate
Ki(μM) 1.37 ± 0.23 7.44 ± 0.84
KI(μM) 0.72 ± 0.11 1.12 ± 0.11
kinact (s−1) 0.015 ± 0.002 0.015 ± 0.002
kinact/KI (μM−1 s−1) 0.021 ± 0.004 0.013 ± 0.002
kcat/kinact (tn) 40 ± 10 50 ± 10
kcat (s−1) 0.60 ± 0.17 0.75 ± 0.18
Achiral Cephalothin BATSI
Ki(μM) 42 ± 4 36 ± 4
Chiral Cephalothin BATSI
Ki(μM) 0.68 ± 0.07 3.8 ± 0.4
Methylidene Penem
Ki(μM) 0.057 ± 0.006 0.102 ± 0.001
Ki(μM) 0.026 ± 0.004 0.039 ± 0.002
kinact (s−1) 0.17 ± 0.01 0.24 ± 0.02
kinact/KI (μM−1 s−1) 6.5 ± 1.0 6.2 ± 0.6
kcat/kinact (tn) 2 ± 1 2 ± 1
kcat (s−1) 0.34 ± 0.17 0.48 ± 0.24
Meropenem
Ki(μM) 31 ± 3 1411 ± 14