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. Author manuscript; available in PMC: 2014 May 22.
Published in final edited form as: Cancer Res. 2013 Jan 15;73(6):1922–1933. doi: 10.1158/0008-5472.CAN-12-3175

Figure 3. S3I-1757 but not S3I-1756 induces nuclear accumulation of P-STAT3 and inhibits STAT3-DNA binding.

Figure 3

(A and B) MDA-MB-468 cells were plated on cover slides over night and then treated with either vehicle, S3I-1757 or S3I-1756 at the indicated concentration for either 2 or 4 hours (A) or 18 hours (B) and processed for P-STAT3 immuno-fluorescence as described in Methods. (C) MDA-MB-468 and A-549 cells were treated with either vehicle, S3I-1757 or S3I-1756 for the indicated interval of time and the nuclear extract isolated from the treated cells as described in the Methods. The nuclear extracts were then incubated with a biotin labeled STAT3 DNA binding probe and the complexes isolated by a STAT3-DNA binding assay described under Methods. Results are representative of 4 independent experiments.