Figure 7. S3I-1757, but not S3I-1756, inhibits migration and invasion selectively in human cancers that harbor high levels of P-STAT3.

(A) Migration: MDA-MB-231, A549 and H460 cells were seeded and allowed to grow overnight prior to scratching the cells with pipette tips. Cells were then treated with vehicle, S3I-1756 or S3I-1757 and allowed to migrate into the scratched area for 16 hours in regular growth medium as described in Methods. (B) Invasion: H460, A549, MDA-MB-468 and MDA-MB-231 cells were seeded over the Matrigel in the top chamber of insert, and treated with vehicle, S3I-1756, or S3I-1757 the following day. The cells were incubated for 48 hours, and the invaded cells were fixed with methanol, stained with crystal violet and photographed as described under Methods. Experiments were performed in triplicates with identical results.